Amandine Henry scored a dazzling curled effort in the sixth minute, before Ada Hegerberg headed home an exquisite Selma Bacha cross to double Lyon’s lead.
Catarina Macario tapped home a third from close range before Alexia Putellas pulled one back for the Spanish giants.
Despite pressure from Barca in the second half, Lyon remained resolute, once again being crowned European champions.
It was the battle of the two biggest teams in women’s football.
Lyon: The seven-time winners packed full of experienced winners and led by prolific goal scorer Hegerberg.